NCAPH 抗体 (AA 440-457)
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Quick Overview for NCAPH 抗体 (AA 440-457) (ABIN401274)
抗原
See all NCAPH 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 440-457
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特异性
- This antibody reacts against BRRN1 protein.
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交叉反应 （详细）
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Species reactivity (expected):Chimpanzee, Mouse, Rat, Canine, Chicken.
Species reactivity (tested):Human. -
纯化方法
- Immunoaffinity chromatography
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免疫原
- Synthetic peptide corresponding to amino acids 440-457 of Human BRRN-1
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亚型
- IgG
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anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (AA 645-741) antibody
NCAPH 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C9 unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) antibodyNCAPH 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (AA 1-250) antibodyNCAPH 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (AA 354-382) antibodyNCAPH 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB39349 unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (AA 601-700) antibodyNCAPH 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (N-Term) antibodyNCAPH 适用: 人, 大鼠, 小鼠, Cow, 犬, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (AA 1-741) antibodyNCAPH 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (N-Term) antibodyNCAPH 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) (C-Term) antibodyNCAPH 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Non-SMC Condensin I Complex, Subunit H (NCAPH) antibodyNCAPH 适用: 人 DB 宿主: 小鼠 Monoclonal 2094C4_2a unconjugated
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应用备注
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ELISA: 1/8000 - 1/35000. Western Blot: 1/500 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.93 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- NCAPH (Non-SMC Condensin I Complex, Subunit H (NCAPH))
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别名
- NCAPH
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背景
- BRNN1, also called Barren, Condensin subunit 2, Barren homolog protein 1, Chromosomeassociated protein H, hCAP-H, and XCAP-H homolog, is encoded by a gene that is a member of the barr gene family. Brnn1 is the regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. The condensin complex contains the SMC2L1 and SMC4L1 heterodimer, and three non SMC subunits that probably regulate the complex: BRRN1/CAPH, CNAP1/CAPD2 and CAPG. BRRN1 has both a nuclear and cytoplasmic localization. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2 leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. BRRN1 is widely expressed at low level in most proliferating cells. CDC2 phosphorylates BRRN1. Its phosphorylation, as well as that of CNAP1 and CAPG subunits, activates the condensin complex and is required for chromosome condensation.Synonyms: BRRN, BRRN1, CAPH, Chromosome-associated protein H, KIAA0074
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基因ID
- 23397
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NCBI登录号
- NP_056156
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UniProt
- Q15003
抗原
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