This antibody is directed against human PACRG protein. Expect reactivity with both splice variants of this protein.
交叉反应 (详细)
Species reactivity (expected):Mouse and chicken (100 %).Cross reactivity with PACRG protein from zebrafish and rat may occur as this sequence only varies by one amino acid residue (94 % homology). Species reactivity (tested):Human.
纯化方法
Affinity chromstography
免疫原
Synthetic peptide corresponding to amino acids 204-215 of Human PACRG protein
ELISA: 1: 2,000 - 1: 10,000. Western blot: 1: 500 - 1: 3,000 (Expect a band approx. 40 kDa in size corresponding to PACRG inthe appropriate cell lysate or extract). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.6 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
PACRG (also known as Parkin coregulated gene protein and PARK2 coregulated) is a gene located very close to parkin, in reverse orientation on the chromosome. It is thought to be cotranscribed with parkin by a bi-directional promoter between the two genes. PACRG is expressed in all immune tissues, spleen, lymph nodes, thymus, tonsils, leukocyte and bone marrow and is also expressed in heart, brain, skeletal muscle, kidney, lung and pancreas. PACRG is expressed in primary Schwann cells and very weakly by monocyte-derived macrophages, which are the primary host cells of Mycobacterium leprae, the causative agent of leprosy. Splice variants have been described for this protein.Synonyms: GLUP, Molecular chaperone/chaperonin-binding protein, PARK2 coregulated gene protein, PARK2CRG, Parkin coregulated gene protein