R-Phycoerythrin 抗体 (Texas Red (TR))
The Texas Red (TR)-conjugated 山羊 多克隆 anti-R-Phycoerythrin antibody (ABIN401163) specifically detects R-Phycoerythrin in FACS 和 IF.
产品编号 ABIN401163
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
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Quick Overview for R-Phycoerythrin 抗体 (Texas Red (TR)) (ABIN401163)
抗原
R-Phycoerythrin
(Phycoerythrin, R-)
宿主
山羊
克隆类型
多克隆
标记
This R-Phycoerythrin antibody is conjugated to Texas Red (TR)
应用范围
Flow Cytometry (FACS), Immunofluorescence (IF)
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产品特性
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Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 2.5 Moles Texas Red(TM) per mole of Goat IgG -
纯化方法
- Immunoaffinity Chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
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免疫原
- Highly purified R-Phycoerythrin from the seaweed gracila
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亚型
- IgG
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anti-Phycoerythrin, R- antibody (Alkaline Phosphatase (AP))
WB, ELISA, IHC 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
anti-Phycoerythrin, R- antibody (Texas Red (TR))WB, DB, FM 宿主: 山羊 Polyclonal Texas Red (TR)
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应用备注
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This product is designed for immunofluorescence microscopy, fluorescence based plateassays (FLISA) and fluorescent western blotting. This product is also suitable for multiplexanalysis, including multicolor imaging, utilizing various commercial platforms. Immunofluorescence: 1/500-1/2500. Flow Cytometry: 1/2000-1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent) For extended storage, restore with 50 % glycerol
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- R-Phycoerythrin (Phycoerythrin, R-)
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别名
- R-Phycoerythrin
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物质类
- Chemical
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背景
- Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB
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基因ID
- 2775
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UniProt
- Q7SIF9
抗原
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