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R-Phycoerythrin 抗体 (HRP)

The HRP-conjugated 山羊 多克隆 anti-R-Phycoerythrin antibody (ABIN401160) specifically detects R-Phycoerythrin in WB, IHC (fro), DB 和 EIA.
产品编号 ABIN401160
发货至: 中国
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Quick Overview for R-Phycoerythrin 抗体 (HRP) (ABIN401160)

抗原

R-Phycoerythrin (Phycoerythrin, R-)

宿主

  • 12
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山羊

克隆类型

  • 15
多克隆

标记

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This R-Phycoerythrin antibody is conjugated to HRP

应用范围

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Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB), Enzyme Immunoassay (EIA)
  • 纯化方法

    Immunoaffinity Chromatography

    免疫原

    Highly purified R-Phycoerythrin from the seaweed gracila

    亚型

    IgG
  • 应用备注

    Western Blot: 1/2000 - 1/10000. Dot Blot. ELISA: 1/300000. Immunohistochemistry: 1/500 - 1/2000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 溶解方式

    Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %

    浓度

    1.0 mg/mL (by UV absorbance at 280 nm)

    缓冲液

    0.02 MPotassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Thimerosal as preservative

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原

    R-Phycoerythrin (Phycoerythrin, R-)

    别名

    R-Phycoerythrin

    物质类

    Chemical

    背景

    Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB

    基因ID

    2775

    UniProt

    Q7SIF9
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