R-Phycoerythrin 抗体 (Biotin)
This 山羊 多克隆 antibody specifically detects R-Phycoerythrin in WB, IHC (fro), DB 和 EIA.
产品编号 ABIN401158
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
Quick Overview for R-Phycoerythrin 抗体 (Biotin) (ABIN401158)
抗原
R-Phycoerythrin
(Phycoerythrin, R-)
宿主
山羊
克隆类型
多克隆
标记
This R-Phycoerythrin antibody is conjugated to Biotin
应用范围
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB), Enzyme Immunoassay (EIA)
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产品特性
- Molar Ratio: 10-20 BAC molecules per Goat IgG molecule
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纯化方法
- Immunoaffinity Chromatography using R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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免疫原
- Highly purified R-Phycoerythrin from the seaweed gracila
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亚型
- IgG
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anti-Phycoerythrin, R- antibody (Alkaline Phosphatase (AP))
WB, ELISA, IHC 宿主: 山羊 Polyclonal Alkaline Phosphatase (AP)
anti-Phycoerythrin, R- antibody (Texas Red (TR))WB, DB, FM 宿主: 山羊 Polyclonal Texas Red (TR)
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应用备注
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Suitable for Immunoblotting (western or dot blot), ELISA as well as other Immunochemicalassays. R-Phycoerythrin (240 kDa) is a labile molecule that may dissociate intocomponents upon exposure to reducing or denaturing agents. Reaction with low molecularweight fragments is typically noted by western blot. Recommneded Dilutions: ELISA: 1/200,000. Western Blot: 1/2000-1/20,000. Immunohistochemistry: 1/500-1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent). For extended storage add glycerol to 50 %
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- R-Phycoerythrin (Phycoerythrin, R-)
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别名
- R-Phycoerythrin
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物质类
- Chemical
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背景
- Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB
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基因ID
- 2775
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UniProt
- Q7SIF9
抗原
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