IL27 抗体 (HRP)
Quick Overview for IL27 抗体 (HRP) (ABIN401111)
抗原
See all IL27 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody reacts to Interleukin-27A.
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交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse. -
纯化方法
- Ion exchange chromatography
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免疫原
- Full length recombinant mouse IL27/p28 protein
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亚型
- IgG
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anti-Interleukin-27 Subunit alpha (IL27) antibody
IL27 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 Subunit alpha (IL27) (AA 1-173) antibodyIL27 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 Subunit alpha (IL27) (AA 29-234) antibodyIL27 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 Subunit alpha (IL27) (AA 32-234) antibodyIL27 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 Subunit alpha (IL27) (N-Term) antibodyIL27 适用: 人, 小鼠 WB, EIA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin-27 Subunit alpha (IL27) antibody (FITC)IL27 适用: 人 FACS 宿主: 小鼠 Monoclonal B-G49 FITC
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应用备注
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ELISA: 1/10,000 - 1/50,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 1/500 - 1/2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative (Do NOT add Sodium Azide!)
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- IL27 (Interleukin-27 Subunit alpha (IL27))
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别名
- Interleukin-27 / IL27A
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背景
- The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.Synonyms: IL-27 subunit alpha, IL-30, IL27-A, IL30, Interleukin-30, p28
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基因ID
- 246779
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NCBI登录号
- NP_663611
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UniProt
- Q8K3I6
抗原
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