ATPase (Na/K) 抗体
The 小鸡 多克隆 anti-ATPase (Na/K) antibody (ABIN401097) specifically detects ATPase (Na/K) in WB, IHC (fro) 和 EIA.
The antibody is reactive with 犬 samples.
产品编号 ABIN401097
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for ATPase (Na/K) 抗体 (ABIN401097)
抗原
ATPase (Na/K)
适用
犬
宿主
小鸡
克隆类型
多克隆
标记
This ATPase (Na/K) antibody is un-conjugated
应用范围
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
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特异性
- This antibody reacts to Sodium Potassium ATPase.
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纯化方法
- Ion exchange chromatography
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免疫原
- ATPase, Sodium, Potassium [Canine Kidney]
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亚型
- Ig Fraction
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anti-ATPase (Na/K) antibody
适用: 人, 大鼠, 小鼠 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATPase (Na/K) (AA 12-46) antibody适用: 人 WB 宿主: 兔 Polyclonal RB55974 unconjugated
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应用备注
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Western Blot: 1/100 to 1/500. ELISA: 1/1,000 to 1/6,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
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浓度
- 10.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- ATPase (Na/K)
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背景
- The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP dependent transport of sodium and potassium across the cell membrane. This membrane bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium / potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium / potassium ATPase exhibit tissue specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell specific expression. The alpha subunit has been found in kidney, brain, heart, and to a lesser extent liver, skeletal and smooth muscle.Synonyms: ATPase Sodium/Potasium, Sodium pump 1, Sodium/potassium dependent ATPase
抗原
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