L1/ORF2 抗体
The 小鸡 多克隆 anti-L1/ORF2 antibody is suitable to detect L1/ORF2 in samples from Chimpanzee 和 人. It has been validated for EIA 和 WB.
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产品编号 ABIN401096
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for L1/ORF2 抗体 (ABIN401096)
抗原
L1/ORF2
适用
Chimpanzee, 人
宿主
小鸡
克隆类型
多克隆
标记
非结合性
应用范围
Enzyme Immunoassay (EIA), Western Blotting (WB)
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交叉反应 （详细）
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Species reactivity (expected):Chimpanzee.
Species reactivity (tested):Human. -
纯化方法
- IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer.
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免疫原
- Two Synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein
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亚型
- IgG
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应用备注
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ELISA: 1/10000. Western Blot: 1/2000 - 1/10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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- L1/ORF2
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别名
- L1 / ORF2
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背景
- The L1 (LINE-1), or Long INterspersed Element, retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retrotransposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.Synonyms: LINE-1
抗原
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