Protein G 抗体 (HRP)
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北京 101111
Quick Overview for Protein G 抗体 (HRP) (ABIN401071)
抗原
See all Protein G 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Ion exchange chromatography
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免疫原
- Protein G [Streptococcus species]
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亚型
- IgG
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anti-Protein G antibody (HRP)
适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal HRP
anti-Protein G antibody (Biotin)适用: Streptococcus ELISA, DB 宿主: 兔 Polyclonal Biotin
anti-Protein G antibody (FITC)适用: Streptococcus FACS, IHC 宿主: 兔 Polyclonal FITC
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应用备注
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Western Blot. Dot Blot. ELISA: 1/20000 to 1/100000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 2.0 mL of deionized water (or equivalent). For extended storage reconstitute product with 50 % glycerol.
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浓度
- 10.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative
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储存液
- Gentamicin sulfate
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注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Protein G
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背景
- Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but unlike Protein A can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
抗原
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