SAE1 抗体
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Quick Overview for SAE1 抗体 (ABIN401032)
抗原
See all SAE1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody is directed against SUMO Activating Enzyme E1 protein.
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交叉反应 （详细）
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Species reactivity (expected):Bovine, Canine, Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography on Protein A
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免疫原
- Recombinant protein produced by baculoviral expression in insect cells (Sf9, Spodoptera frugiperda) corresponding to full length Human SUMO Activating Enzyme E1 fused with GST
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亚型
- IgG
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anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibody
SAE1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1G4-1G5 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodyKO Validated SAE1 适用: 人 WB, IF, IHC (p), ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (Internal Region) antibodySAE1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, ICC, FACS 宿主: 兔 Monoclonal 24GB1565 unconjugated Recombinant Antibody
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibodySAE1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 300-329), (C-Term) antibodySAE1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB2887-RB2888 unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) antibodySAE1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 9D8 unconjugated Recombinant Antibody
anti-SUMO1 Activating Enzyme Subunit 1 (SAE1) antibodySAE1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB5825 unconjugated
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应用备注
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ELISA: 1/5,000 - 1/20,000. Western Blot: 1/500 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water or equivalent.
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浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide, None
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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别名
- SAE1 / AOS1
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背景
- SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) is a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitin-like SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1, also called Aos1) subunit resembles the Nterminal half of yeast UBA1, the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.Synonyms: SUA1, SUMO-activating enzyme subunit 1, UBLE1A, Ubiquitin-like 1-activating enzyme E1A
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基因ID
- 10055
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NCBI登录号
- NP_005491
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UniProt
- Q9UBE0
抗原
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