CD97 抗体 (Extracellular Domain, N-Term)
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- 抗原 See all CD97 抗体
- CD97
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抗原表位
- AA 1-512, Extracellular Domain, N-Term
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CD97 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against the CD97 protein. A BLAST analysis was used to suggest cross reactivity with CD97 proteins from Mouse (100 %) and Rat (74 %). Only 49 % homology is noted for the Human homologue. Reactivity against CD97 from other sources is not known.
- 交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse, Human, Chimpanzee. - 纯化方法
- Affinity chromatography on Protein A
- 免疫原
- Recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein
- 亚型
- IgG
- Top Product
- Discover our top product CD97 Primary Antibody
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anti-CD97 (CD97) (AA 419-552) antibody
CD97 适用: 人 WB, IHC, ELISA, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 6D7E6 unconjugated
anti-CD97 (CD97) (AA 741-835) antibodyCD97 适用: 人 ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) (AA 421-529) antibodyCD97 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 5D5 unconjugated
anti-CD97 (CD97) (AA 58-260) antibodyCD97 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-CD97 (CD97) (AA 240-435) antibodyCD97 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) antibodyCD97 适用: 人 IHC, ELISA, IF 宿主: 兔 Monoclonal 3A7 unconjugated Recombinant Antibody
anti-CD97 (CD97) (N-Term) antibodyCD97 适用: 人 IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) antibody (FITC)CD97 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-180 FITC
anti-CD97 (CD97) (AA 32-173) antibodyCD97 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD97 (CD97) (AA 421-529) antibodyCD97 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
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Immunofluorescence: 1/1000 - 1/5000. Western Blot: 1/1000 - 1/5000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL deionized water (or equivalent).
- 浓度
- 5.0 mg/mL (UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M NaCl, pH 7.2, 0.01 % (w/v) Sodium Azide, None
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- CD97
- 别名
- CD97 (CD97 产品)
- 别名
- AA409984 antibody, TM7LN1 antibody, adhesion G protein-coupled receptor E5 antibody, Adgre5 antibody, ADGRE5 antibody
- 背景
- CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including hematopoietic cells. Particularly high levels of expression are also seen in the lung and the thymus gland. In the spleen, expression is detected on most myeloid cells and variable portions of T cells, B cells and NK cells. In humans, CD97 expression is upregulated during lymphocyte activation on various epithelial cancer cells including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha 5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Antibodies against CD97 are suitable for exploring various mouse genetic models of cancer, angiogenesis, and inflammation. CD97 can be proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit. Multiple isoforms have been identified for this protein.Synonyms: Leukocyte antigen CD97
- 基因ID
- 26364
- NCBI登录号
- NP_001156501
- UniProt
- Q9Z0M6
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