UBA1 抗体
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Quick Overview for UBA1 抗体 (ABIN401028)
抗原
See all UBA1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody is directed against Ubiquitin Activating Enzyme E1 protein.
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交叉反应 （详细）
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Species reactivity (expected):Rabbit, Mouse, Rat, Canine.
Species reactivity (tested):Human. -
纯化方法
- Purified
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免疫原
- Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST
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亚型
- IgG
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anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 300-600) antibody
UBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 1-1058) antibodyUBA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 730-881) antibodyUBA1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 1008-1058) antibodyUBA1 适用: 人, 小鼠 WB, IP, IHC (fp) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyKD Validated UBA1 适用: 人 WB 宿主: 兔 Monoclonal 25GB7860 unconjugated Recombinant Antibody
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 961-1058) antibodyUBA1 适用: 人, 大鼠 ELISA, IF (cc), IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 800-850) antibodyUBA1 适用: 人 IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (N-Term) antibodyUBA1 适用: 人, 大鼠, 小鼠, 犬, Cow, 马, 兔, 豚鼠, Bat, Hamster, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Reconstitute with 0.1 mL of deionized water or equivalent.
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浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
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别名
- UBE1 / UBA1
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背景
- Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1
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基因ID
- 7317
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NCBI登录号
- NP_003325
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UniProt
- P22314
抗原
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