UBA1 抗体
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- 抗原 See all UBA1 抗体
- UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
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适用
- 人, 大鼠, 小鼠, 犬, 兔
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This UBA1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against Ubiquitin Activating Enzyme E1 protein.
- 交叉反应 （详细）
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Species reactivity (expected):Rabbit, Mouse, Rat, Canine.
Species reactivity (tested):Human. - 纯化方法
- Purified
- 免疫原
- Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST
- 亚型
- IgG
- Top Product
- Discover our top product UBA1 Primary Antibody
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anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 300-600) antibody
UBA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人, 大鼠, 小鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 1-1058) antibodyUBA1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (C-Term) antibodyUBA1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 730-881) antibodyUBA1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibodyUBA1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (AA 961-1058) antibodyUBA1 适用: 人, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (N-Term) antibodyUBA1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (N-Term) antibodyUBA1 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/2,000 - 1/10,000. Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry: 2 μg/mL - 20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Reconstitute with 0.1 mL of deionized water or equivalent.
- 浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))
- 别名
- UBE1 / UBA1 (UBA1 产品)
- 别名
- A1S9 antibody, A1S9T antibody, A1ST antibody, AMCX1 antibody, GXP1 antibody, POC20 antibody, SMAX2 antibody, UBA1A antibody, UBE1 antibody, UBE1X antibody, DDBDRAFT_0190927 antibody, DDBDRAFT_0220497 antibody, DDB_0190927 antibody, DDB_0220497 antibody, Sbx antibody, Ube-1 antibody, Ube1x antibody, a1s9 antibody, a1s9t antibody, a1st antibody, amcx1 antibody, gxp1 antibody, poc20 antibody, smax2 antibody, uba1a antibody, ube1 antibody, ube1x antibody, uba1 antibody, uba1b antibody, ube antibody, zgc:66143 antibody, wu:fa01e08 antibody, wu:fb30f01 antibody, wu:fi21c11 antibody, wu:fj14g11 antibody, ubiquitin like modifier activating enzyme 1 antibody, ubiquitin activating enzyme E1 antibody, Ubiquitin-conjugating enzyme E1 antibody, ubiquitin-like modifier activating enzyme 1 antibody, ubiquitin-like modifier activating enzyme 1 S homeolog antibody, ubiquitin-like modifier activating enzyme 1 L homeolog antibody, UBA1 antibody, uae1 antibody, GL50803_4083 antibody, GL50803_10661 antibody, Uba1 antibody, uba1.S antibody, uba1.L antibody, uba1 antibody, ptr3 antibody
- 背景
- Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitinprotein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a highenergy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 Molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1
- 基因ID
- 7317
- NCBI登录号
- NP_003325
- UniProt
- P22314
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