S100A1 抗体
Quick Overview for S100A1 抗体 (ABIN401026)
抗原
See all S100A1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody is directed against S100 protein.
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交叉反应 （详细）
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Species reactivity (expected):Primate, Mouse, Rat, Canine.
Species reactivity (tested):Bovine. -
纯化方法
- Affinity chromatography on Protein A
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免疫原
- Prepared from whole rabbit serum produced by repeated immunizations with fulllength bovine S100 protein (mixture of alphaalpha homodimers and alphabeta heterodimers)
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亚型
- IgG
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anti-S100 Calcium Binding Protein A1 (S100A1) (AA 2-94) antibody
S100A1 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (N-Term) antibodyS100A1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-75) antibodyS100A1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal S100A1-1942 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-94) antibodyS100A1 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-490R unconjugated Recombinant Antibody
anti-S100 Calcium Binding Protein A1 (S100A1) antibodyS100A1 适用: 人 IHC, ELISA 宿主: 小鼠 Monoclonal 2C8B8 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-94) antibodyS100A1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-94) antibodyS100A1 适用: 人 WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C8 unconjugated
anti-S100 Calcium Binding Protein A1 (S100A1) (AA 1-94) antibodyS100A1 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/3,000. Immunohistochemistry: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 0.1 mL of deionized water (or equivalent).
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浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- S100A1 (S100 Calcium Binding Protein A1 (S100A1))
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别名
- S100A1
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背景
- S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21 kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - betabeta homodimer (S-100b) and alphabeta heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke. Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.Synonyms: S-100 protein alpha chain, S-100 protein subunit alpha, S100 alpha, S100 calcium-binding protein A1, S100-A1, S100A
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基因ID
- 528735
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NCBI登录号
- NP_001092512
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UniProt
- P02639
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途径
- Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins
抗原
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