NRF1 抗体 (N-Term)
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北京 101111
Quick Overview for NRF1 抗体 (N-Term) (ABIN401025)
抗原
See all NRF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-534, N-Term
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交叉反应 （详细）
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Species reactivity (expected):Human, Chimpanzee, Canine, Rat, Chicken, Frog, Zebrafish.
Species reactivity (tested):Mouse. -
纯化方法
- Affinity Chromatography on Protein A.
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免疫原
- Recombinant Mouse NRF1 protein corresponding to aa 1-534 of the native protein. Genename: NRF1
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亚型
- IgG
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anti-Nuclear Respiratory Factor 1 (NRF1) (AA 201-285) antibody
NRF1 适用: 人 WB, ELISA, IHC (p), RNAi 宿主: 小鼠 Monoclonal 2F9 unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) antibodyNRF1 适用: 人 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 272-288), (Middle Region) antibodyNRF1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (Middle Region) antibodyNRF1 适用: 人, 小鼠, 大鼠, Cow, 兔, 马, 豚鼠, 斑马鱼, 犬 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) antibodyNRF1 适用: 人 WB, IHC, IP, ChIP 宿主: 兔 Monoclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 51-180) antibodyNRF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 360-409) antibodyNRF1 适用: 人, 大鼠, Cow, 兔, 马, 豚鼠, 猴, 斑马鱼, 小鸡, 犬, Pig, 非洲爪蟾 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 1-300) antibodyNRF1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 201-285) antibodyNRF1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Nuclear Respiratory Factor 1 (NRF1) (AA 429-478) antibodyNRF1 适用: 人, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/3000-1/10000. Western Blot: 1/500-1/2000. Immunohistochemistry on Paraffin Sections: 2 mg/mL-5 μg/mL. Expect a band approx. 67 kDa in size corresponding to NRF1 by Western blotting in theappropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of Lower MolecularWeight bands.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 2.3 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody (in aliquots) at-20 °C. Dilute only prior to immediate use.
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- NRF1 (Nuclear Respiratory Factor 1 (NRF1))
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别名
- NRF1
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背景
- NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. NRF1 forms a homodimer and binds DNA as a dimer. NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues. Phosphorylation of NRF1 enhances DNA binding. Multiple splice variants have been identified for this protein.Synonyms: Alpha palindromic-binding protein, Alpha-pal, NRF-1, Nuclear respiratory factor 1
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基因ID
- 10090
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UniProt
- Q9WU00
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途径
- Regulation of Lipid Metabolism by PPARalpha
抗原
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