MBP Tag 抗体
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北京 101111
Quick Overview for MBP Tag 抗体 (ABIN401023)
抗原
宿主
克隆类型
标记
应用范围
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特异性
- This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays. This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag. To date this antibody has reacted with all MBP tagged proteins so far tested. In western blotting of bacterial extracts the antibody does not cross-react with endogenous proteins.
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纯化方法
- Purified
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免疫原
- Whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein
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亚型
- IgG
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anti-MBP Tag antibody
WB 宿主: 兔 Polyclonal RB55937 unconjugated
anti-MBP Tag antibody适用: 标记 WB, ELISA, IP, ICC, IHC, FACS 宿主: 小鼠 Monoclonal 3G1A3 unconjugated
anti-MBP Tag antibodyWB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-MBP Tag antibodyWB 宿主: 小鼠 Monoclonal 8K2 unconjugated
anti-MBP Tag antibodyWB, ELISA 宿主: 小鼠 Monoclonal 5B10B11 unconjugated
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应用备注
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Western blot: 1/3500. ELISA: 1/4000 to 1/16000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
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浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
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缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- MBP Tag
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别名
- Maltose Binding Protein Tag / MBP-Tag
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物质类
- Tag
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背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag
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基因ID
- 948538
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NCBI登录号
- AP_004535
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UniProt
- P0AEX9
抗原
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