CD3 抗体 (Activation Site)
Quick Overview for CD3 抗体 (Activation Site) (ABIN400888)
抗原
See all CD3 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Activation Site
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特异性
- The antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
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交叉反应 （详细）
- Species reactivity (tested):Human, Mouse
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产品特性
- Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4
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纯化方法
- Protein A affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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免疫原
- Purified human CD3 proteins isolated from thymus
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亚型
- IgG1
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anti-CD3 (CD3) antibody
CD3 适用: 人, 小鼠, 大鼠 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), ICC, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro), Func, MS 宿主: 小鼠 Monoclonal UCHT1 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, Func, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibodyCD3 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-57 unconjugated
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal UCHT1 Biotin
anti-CD3 (CD3) antibody (Biotin)CD3 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-57 Biotin
anti-CD3 (CD3) antibody (PerCP)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PerCP
anti-CD3 (CD3) antibody (APC)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 APC
anti-CD3 (CD3) antibody (PE)CD3 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal UCHT1 PE
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应用备注
- Flow Cytometry. Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS+ 0.1 % BSA for 20 min. on ice. Wash with PBS and fix with 2 % formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0,1 % saponine, 5 min. RT. Incubate the cells in PBS + 1 % BSA + 0.03 % saponine, 15 min. on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-5 μg/mL) in PBS + 1 % BSA + 0.03 %saponine in dark, 20 min. RT. Wash with PBS + 1 % BSA + 0.03 % saponine, resuspend in PBS. Positive control: Human T cells stimulated with anti-CD3 (MEM-57) antibody (1 μg/mL). Immunoprecipitation.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS, pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- CD3
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别名
- CD3
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背景
- CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
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基因ID
- 916
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UniProt
- P07766
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途径
- TCR Signaling, Ubiquitin Proteasome Pathway
抗原
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