CRE Recombinase (CRE) 抗体
This 小鼠 单克隆 antibody specifically detects CRE Recombinase in WB 和 ELISA. It exhibits reactivity toward Bacteriophage P1. It has been mentioned in 2+ publications
1 image
(2 references)
产品编号 ABIN398566
发货至:
中国
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Quick Overview for CRE Recombinase (CRE) 抗体 (ABIN398566)
抗原
See all CRE Recombinase (CRE) 抗体
CRE Recombinase (CRE)
适用
Bacteriophage P1
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Western Blotting (WB), ELISA
克隆位点
1D8H3
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特异性
- Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.
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交叉反应 （详细）
- Negative Control: E. coli. and cell lysate (not mammalian) It's not suitable for the analysis of mammalian cell lysate.
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纯化方法
- Protein A affinity column
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免疫原
- Purified recombinant Cre recombinase protein
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亚型
- IgG2a
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anti-CRE Recombinase (CRE) antibody
CRE 适用: Bacteriophage P1 WB 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Working concentrations for specific applications should be determined by the investigator. The chart below may serve as a useful guideline, but secondary antibody affinity, antigen concentration, temperature, incubation time, and the sensitivity of the detection methods can all affect the ideal working concentration. We have not yet determined the suitability of this antibody for other applications. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1.0 µg/mL Other applications: user-optimized -
限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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储存条件
- 4 °C/-20 °C
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储存方法
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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: "Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line." in: In vitro cellular & developmental biology. Animal, Vol. 49, Issue 2, pp. 114-21, (2013) (PubMed).
: "Loss of autophagy in hypothalamic POMC neurons impairs lipolysis." in: EMBO reports, Vol. 13, Issue 3, pp. 258-65, (2012) (PubMed).
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: "Establishment and characterization of a tamoxifen-mediated reversible immortalized mouse dental papilla cell line." in: In vitro cellular & developmental biology. Animal, Vol. 49, Issue 2, pp. 114-21, (2013) (PubMed).
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- CRE Recombinase (CRE)
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别名
- Cre Recombinase
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物质类
- Phage Protein
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背景
- Cre-recombinase is a 38 kDa DNA recombinase derived from the P1 bacteriophage. It is highly specific to a 34 bp DNA sequence (loxP) found in P1 DNA. It catalyzes site-specific recombination between two 34-base-pair LOXP sites. Its role is to maintain the phage genome as a monomeric unit-copy plasmid in the lysogenic state. It is homotetramer when bound to DNA and belongs to the phage integrase family.Mouse Anti-Cre recombinase Monoclonal Antibody is an important tool for the identification of Cre recombinase in different protein expression systems.
抗原
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