HBxAg 抗体
The 小鼠 单克隆 anti-HBxAg antibody (Clone 3F6-G10) (ABIN393198) specifically detects HBxAg in WB, ELISA, IHC (fro), IHC (p) 和 IHC.
The antibody is reactive with Hepatitis B Virus (HBV) samples.
产品编号 ABIN393198
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for HBxAg 抗体 (ABIN393198)
抗原
HBxAg
(Hepatitis B Virus X Antigen (HBxAg))
适用
Hepatitis B Virus (HBV)
宿主
小鼠
克隆类型
单克隆
标记
This HBxAg antibody is un-conjugated
应用范围
Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
克隆位点
3F6-G10
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特异性
- Recognizes the HB-X antigen of hepatitis virus. The Hepatitis B X antigen is a 154 amino acid ~17 kD multifunctional protein involved in the development of liver cirrhosis and hepatocellular carcinoma. Clone 3F6-G10 was produced by immunization of mice with a "HB-X- Protein A" fusion construct and subsequent screening of hybridoma products against a "HB-X-GST" fusion construct. Has been used successfully for the detection of the hepatitis B X antigen by immunohistochemistry in formalin fixed, paraffin embedded material, also by western blotting against the immunizing and screening fusion proteins. Subsequently clone 3F6-G10 has been used as a capture reagent in a sensitive sandwich ELISA and bead based flow assay for the quantitative assessment of HbX antigen in human sera. Fine epitope mapping by phage library screening indicates that the epitope recognized bylies between amino acids 88 and 93 of the X antigen, a result subsequently confirmed by peptide ELISA.
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纯化方法
- Affinity purified
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免疫原
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HB-Xag-Protein A Fusion protein.
Type of Immunogen: Fusion protein -
亚型
- IgG2a
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anti-Hepatitis B Virus X Antigen (HBxAg) antibody
HBxAg 适用: Hepatitis B Virus (HBV) WB, ELISA, IF (cc), IF (p), IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA, IHC, IHC-Fr (1:100), IHC-P (1:100), WB (1:100 - 1:500)
Usage: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. -
说明
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Target Species of Antibody: Hepatitis B Virus
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.09 % sodium azide, 0.1 % BSA.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing. Store undiluted.
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储存条件
- 4 °C,-20 °C
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储存方法
- 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.
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- HBxAg (Hepatitis B Virus X Antigen (HBxAg))
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别名
- Hepatitis B X Antigen (HBxAg)
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物质类
- Viral Protein
抗原
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