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Vimentin 抗体 (C-Term)

Name: Vimentin 抗体 (C-Term)
SKU: ABIN389212
Availability: OutOfStock

VIM 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB16691 unconjugated

Anti-Vimentin Antibody (C-term) at 1:1000 dilution + U-2OS whole cell lysate Lysates/proteins at 20 μg per lane. (Vimentin 抗体 (C-Term))

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (Vimentin 抗体 (C-Term))

IHC (p)

Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody. (Vimentin 抗体 (C-Term))

Western blot analysis of Vimentin antibody (C-term) (ABIN389212 and ABIN2839367) in NCI- cell line lysates (35 μg/lane). (Vimentin 抗体 (C-Term))

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). (Vimentin 抗体 (C-Term))

FACS

5 images

(2 references)

产品编号 ABIN389212

发货至: 中国

Datasheet as PDF

抗原 See all Vimentin (VIM) 抗体

Vimentin (VIM)
抗原表位
32

18

16

16

16

13

11

8

8

7

4

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 430-457, C-Term
适用
332

126

125

61

58

43

42

36

29

21

20

13

13

8

7

6

6

4

3

2

1

1

1

1

1

1

1
人
宿主
189

129

9

6

4

4

2
兔
Compatible Secondaries
克隆类型
197

145
多克隆
标记
199

19

13

13

11

7

7

7

7

7

7

7

4

4

4

4

4

3

2

2

2

2

2

2

2

1

1
This Vimentin antibody is un-conjugated
应用范围
281

131

98

98

96

64

49

40

39

28

23

20

8

5

4

4

2

2

2

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

X, B, C, Ha, Pr, M, Pig, Rat

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-457 amino acids from the C-terminal region of human Vimentin.

克隆位点

RB16691

亚型

Ig Fraction

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VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) (AA 210-466) antibody
VIM 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 5D7 unconjugated

应用备注

IF: 1:100. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Liu, Li, Li, Yang: "Pituitary tumor transforming gene PTTG2 induces psoriasis by regulating vimentin and E-cadherin expression." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10887-93, (2016) (PubMed).

Dai, Duan, Duan, Zhou, He, Tu, Shen: "Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer." in: Biochemical and biophysical research communications, (2015) (PubMed).
抗原

Vimentin (VIM)

别名

Vimentin (VIM 产品)

别名

CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody

背景

Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.

分子量

53652

基因ID

7431

NCBI登录号

NP_003371

UniProt

P08670

途径

Caspase Cascade in Apoptosis