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SYVN1 抗体 (C-Term)

Cited in 12+ publications. The 兔多克隆 anti-SYVN1 antibody (Clone RB4822) (ABIN388980) specifically detects SYVN1 in WB, IF 和 IHC (p). The antibody is reactive with 人和小鼠 samples.

Fluorescent confocal image of HeLa cells stained with SYVN1 (HRD1) (C-term) antibody. (SYVN1 抗体 (C-Term))

Mouse Neuroblastoma Neuro2A (N2A) was transiently transfected, collected at 72h after transfection. (SYVN1 抗体 (C-Term))

All lanes : Anti-HRD1 Antibody at 1:2000 dilution Lane 1: D whole cell lysate Lane 2: Hela whole cell lysate Lane 3: mouse spleen lysate Lane 4: PC-3 whole cell lysate Lysates/proteins at 20 μg per lane. (SYVN1 抗体 (C-Term))

Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term) (ABIN388980 and ABIN2837882) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (SYVN1 抗体 (C-Term))

IHC (p)

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SYVN1 抗体 (C-Term))

IHC (p)

6 images

(12 references)

产品编号 ABIN388980

发货至: 中国

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Quick Overview for SYVN1 抗体 (C-Term) (ABIN388980)

抗原

See all SYVN1 抗体

SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This SYVN1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB4822

抗原表位
16

7

5

5

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 586-617, C-Term

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This SYVN1 (HRD1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 586-617 amino acids from the C-terminal region of human SYVN1 (HRD1).

亚型

Ig Fraction
anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 586-617) antibody
SYVN1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
Verified SYVN1 适用: 人, 大鼠 WB, ELISA, FACS, IF 宿主: 山羊 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 531-617) antibody
SYVN1 适用: 人, 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
SYVN1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 338-487) antibody
SYVN1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
SYVN1 适用: 人, 小鼠, 大鼠, 马, 犬, Cow, 兔, Bat, 猴, Hamster, 非洲爪蟾, 斑马鱼 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 1-617) antibody
SYVN1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 238-318) antibody
SYVN1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H4 unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (Internal Region) antibody
Verified SYVN1 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1) (AA 371-420) antibody
SYVN1 适用: 人, 小鼠, 大鼠, 马, 犬, 豚鼠, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:200. WB: 1:2000. WB: 1:2000. WB: 1:1000. IHC-P: 1:50~100. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze-thaw cycles.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

有效期

6 months
Peters, Déry, LeBlanc: "Familial prion protein mutants inhibit Hrd1-mediated retrotranslocation of misfolded proteins by depleting misfolded protein sensor BiP." in: Human molecular genetics, Vol. 25, Issue 5, pp. 976-88, (2016) (PubMed).

Jung, Hong, Kim, Mook-Jung: "Acute ER stress regulates amyloid precursor protein processing through ubiquitin-dependent degradation." in: Scientific reports, Vol. 5, pp. 8805, (2015) (PubMed).

Grotzke, Lu, Cresswell: "Deglycosylation-dependent fluorescent proteins provide unique tools for the study of ER-associated degradation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, Issue 9, pp. 3393-8, (2013) (PubMed).

Gharanei, Zatyka, Astuti, Fenton, Sik, Nagy, Barrett: "Vacuolar-type H+-ATPase V1A subunit is a molecular partner of Wolfram syndrome 1 (WFS1) protein, which regulates its expression and stability." in: Human molecular genetics, Vol. 22, Issue 2, pp. 203-17, (2012) (PubMed).

Hibino, Sugiura, Muro, Shimoyama, Tomita: "Cyclosporin A induces the unfolded protein response in keratinocytes." in: Archives of dermatological research, Vol. 303, Issue 7, pp. 481-9, (2011) (PubMed).

Lee, Hammerle, Andrews, Stokes, Mustelin, Silva, Black, Doedens: "Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels." in: The Journal of biological chemistry, Vol. 286, Issue 48, pp. 41530-8, (2011) (PubMed).

Kaneko, Koike, Saito, Kitamura, Okuma, Nomura: "Loss of HRD1-mediated protein degradation causes amyloid precursor protein accumulation and amyloid-beta generation." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 11, pp. 3924-32, (2010) (PubMed).

Saito, Kaneko, Okuma, Nomura: "Correlation between decrease in protein levels of ubiquitin ligase HRD1 and amyloid-beta production." in: Journal of pharmacological sciences, Vol. 113, Issue 3, pp. 285-8, (2010) (PubMed).

Sugiura, Muro, Futamura, Matsumoto, Hashimoto, Nishizawa, Nagasaka, Saito, Tomita, Usukura: "The unfolded protein response is activated in differentiating epidermal keratinocytes." in: The Journal of investigative dermatology, Vol. 129, Issue 9, pp. 2126-35, (2009) (PubMed).

Maeda, Marutani, Zou, Araki, Tanabe, Yagishita, Yamano, Amano, Michikawa, Nakajima, Komano: "An E3 ubiquitin ligase, Synoviolin, is involved in the degradation of immature nicastrin, and regulates the production of amyloid beta-protein." in: The FEBS journal, Vol. 276, Issue 20, pp. 5832-40, (2009) (PubMed).

Toh, Marotte, Blond, Jhumka, Eljaafari, Mougin, Miossec: "Overexpression of synoviolin in peripheral blood and synoviocytes from rheumatoid arthritis patients and continued elevation in nonresponders to infliximab treatment." in: Arthritis and rheumatism, Vol. 54, Issue 7, pp. 2109-18, (2006) (PubMed).

Yamada, Ishihara, Tamura, Takahashi, Yamaguchi, Takei, Tokita, Satake, Tashiro, Katagiri, Aburatani, Miyazaki, Oka: "WFS1-deficiency increases endoplasmic reticulum stress, impairs cell cycle progression and triggers the apoptotic pathway specifically in pancreatic beta-cells." in: Human molecular genetics, Vol. 15, Issue 10, pp. 1600-9, (2006) (PubMed).
抗原

SYVN1 (Synovial Apoptosis Inhibitor 1, Synoviolin (SYVN1))

别名

SYVN1 (HRD1)

背景

HRD1 is a ubiquitin ligase whose expression is induced by the unfolded protein response (UPR) following endoplasmic reticulum stress. Expression of HRD1 protects cells from apoptosis by inducing degradation of abnormally processed proteins that accumulate in the endoplasmic reticulum. HRD1 is expressed in many tissues, strongly expressed in brain, pancreas, liver, kidney and skeletal muscle. Amano T, et al. reported that Synoviolin/Hrd1 (expressed in rheumatoid synovium) is a novel causative factor for arthropathy by triggering synovial cell outgrowth through its antiapoptotic effects. HRD1 contains one ring-type zinc finger.

分子量

67685

基因ID

84447

NCBI登录号

NP_115807, NP_757385

UniProt

Q86TM6

途径

ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling