UCHL5 抗体 (N-Term)
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Quick Overview for UCHL5 抗体 (N-Term) (ABIN388873)
抗原
See all UCHL5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 56-87, N-Term
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预测反应
- B, Pig
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纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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免疫原
- This UCH37 (UCHL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-87 amino acids from the N-terminal region of human UCH37 (UCHL5).
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亚型
- Ig Fraction
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应用备注
- WB: 1:1000. IHC-F: 1:50~100
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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有效期
- 6 months
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- UCHL5 (Ubiquitin Carboxyl-terminal Hydrolase L5 (UCHL5))
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别名
- UCH37 (UCHL5)
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背景
- Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54 % identical to that of UCHL1.
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分子量
- 37607
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基因ID
- 51377
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NCBI登录号
- NP_001186190, NP_001186191, NP_001186192, NP_057068
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UniProt
- Q9Y5K5
抗原
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