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UBE2I 抗体 (N-Term)

Cited in 2+ publications. This 兔 多克隆 anti-UBE2I antibody (Clone RB2811) specifically detects UBE2I in WB 和 IHC (p). The antibody is reactive with 人 samples.
产品编号 ABIN387910
发货至: 中国
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Quick Overview for UBE2I 抗体 (N-Term) (ABIN387910)

抗原

See all UBE2I 抗体
UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

适用

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宿主

  • 74
  • 11
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克隆类型

  • 53
  • 34
多克隆

标记

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This UBE2I antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB2811
  • 抗原表位

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    AA 1-30, N-Term

    预测反应

    Zf, C, M, Rat, X

    纯化方法

    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

    免疫原

    This UBC9 (UBE2I) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UBC9 (UBE2I).

    亚型

    Ig Fraction
  • 应用备注

    WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

    有效期

    6 months
  • Lee, Kraus, Prins, Groenendyk, Aubry, Liu, Li, Julien, Touret, Sykes, Tremblay, Michalak: "UBC9-dependent association between calnexin and protein tyrosine phosphatase 1B (PTP1B) at the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 290, Issue 9, pp. 5725-38, (2015) (PubMed).

    Fu, Xiong, Xu, Cheng, Tang: "MDA5 is SUMOylated by PIAS2? in the upregulation of type I interferon signaling." in: Molecular immunology, Vol. 48, Issue 4, pp. 415-22, (2011) (PubMed).

  • 抗原

    UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

    别名

    UBC9 (UBE2I)

    背景

    UBE2I (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. UBE2I can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase. UBE2I is essential for nuclear architecture and chromosome segregation.

    分子量

    18007

    基因ID

    7329

    NCBI登录号

    NP_003336, NP_919235, NP_919236, NP_919237

    UniProt

    P63279

    途径

    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway
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