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DNMT3A 抗体 (AA 457-486)

Name: DNMT3A 抗体 (AA 457-486)
SKU: ABIN387881
Availability: OutOfStock

This anti-DNMT3A antibody is a 兔多克隆 antibody detecting DNMT3A in WB 和 IHC (p). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 10+ publications.

All lanes : Anti-Dnmt3a Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: Human skeletal muscle tissue lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat heart tissue lysate Lysates/proteins at 20 μg per lane. (DNMT3A 抗体 (AA 457-486))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (DNMT3A 抗体 (AA 457-486))

IHC (p)

All lanes : Anti-Dnmt3a Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: T47D whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: Rat heart tissue lysate Lysates/proteins at 20 μg per lane. (DNMT3A 抗体 (AA 457-486))

Western blot analysis of anti-Dnmt3a Pab (ABIN387881 and ABIN2844092) in T47-D cell lysate. (DNMT3A 抗体 (AA 457-486))

Lysates from mice thymus tissue after radiation were subjected to WB using antibody against DNMT3a. (DNMT3A 抗体 (AA 457-486))

5 images

(10 references)

产品编号 ABIN387881

发货至: 中国

Quick Overview for DNMT3A 抗体 (AA 457-486) (ABIN387881)

抗原

See all DNMT3A 抗体

DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This DNMT3A antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

RB01852

Discover our top product DNMT3A Primary Antibody

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AA 457-486

预测反应

C

纯化方法

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

免疫原

This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.

亚型

Ig Fraction
anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 500-700) antibody
DNMT3A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 26-100) antibody
DNMT3A 适用: 人, 小鼠, 大鼠 FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 46-180) antibody
DNMT3A 适用: 人 ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 4G5F11 unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (2meLys44) antibody
DNMT3A 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 457-486) antibody
DNMT3A 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) antibody
DNMT3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 680-902) antibody
DNMT3A 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 1-912) antibody
DNMT3A 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) (AA 705-908), (C-Term) antibody
DNMT3A 适用: 人, 小鼠 WB, IHC, ICC, ChIP 宿主: 小鼠 Monoclonal 64B1446 unconjugated

anti-DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A) antibody
KO Validated DNMT3A 适用: 人 WB, IP 宿主: 兔 Monoclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Bing, Zhu, Yu, Li, Liu, Li, Wang, Qi, Guo, Yuan, He, Liu, Liu: "Glucocorticoid-induced S-adenosylmethionine enhances the interferon signaling pathway by restoring STAT1 protein methylation in hepatitis B virus-infected cells." in: The Journal of biological chemistry, Vol. 289, Issue 47, pp. 32639-55, (2014) (PubMed).

Siddiqi, Terry, Matushansky: "Hiwi mediated tumorigenesis is associated with DNA hypermethylation." in: PLoS ONE, Vol. 7, Issue 3, pp. e33711, (2012) (PubMed).

Koturbash, Zemp, Kolb, Kovalchuk: "Sex-specific radiation-induced microRNAome responses in the hippocampus, cerebellum and frontal cortex in a mouse model." in: Mutation research, Vol. 722, Issue 2, pp. 114-8, (2011) (PubMed).

Chen, Wang, Hu, Chang, Liao, Dai, Juo: "OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 25, Issue 5, pp. 1718-28, (2011) (PubMed).

Yamagata, Asada, Tamura, Lee, Maekawa, Taniguchi, Taketani, Matsuoka, Tamura, Sugino: "DNA methyltransferase expression in the human endometrium: down-regulation by progesterone and estrogen." in: Human reproduction (Oxford, England), Vol. 24, Issue 5, pp. 1126-32, (2009) (PubMed).

Ishii, Kohu, Yamada, Ishidoya, Kanto, Fuji, Moriya, Satake, Arai: "Up-regulation of DNA-methyltransferase 3A expression is associated with hypomethylation of intron 25 in human testicular germ cell tumors." in: The Tohoku journal of experimental medicine, Vol. 212, Issue 2, pp. 177-90, (2007) (PubMed).

Koturbash, Boyko, Rodriguez-Juarez, McDonald, Tryndyak, Kovalchuk, Pogribny, Kovalchuk: "Role of epigenetic effectors in maintenance of the long-term persistent bystander effect in spleen in vivo." in: Carcinogenesis, Vol. 28, Issue 8, pp. 1831-8, (2007) (PubMed).

Tryndyak, Muskhelishvili, Kovalchuk, Rodriguez-Juarez, Montgomery, Churchwell, Ross, Beland, Pogribny: "Effect of long-term tamoxifen exposure on genotoxic and epigenetic changes in rat liver: implications for tamoxifen-induced hepatocarcinogenesis." in: Carcinogenesis, Vol. 27, Issue 8, pp. 1713-20, (2006) (PubMed).

Koturbash, Rugo, Hendricks, Loree, Thibault, Kutanzi, Pogribny, Yanch, Engelward, Kovalchuk: "Irradiation induces DNA damage and modulates epigenetic effectors in distant bystander tissue in vivo." in: Oncogene, Vol. 25, Issue 31, pp. 4267-75, (2006) (PubMed).

Pogribny, Koturbash, Tryndyak, Hudson, Stevenson, Sedelnikova, Bonner, Kovalchuk: "Fractionated low-dose radiation exposure leads to accumulation of DNA damage and profound alterations in DNA and histone methylation in the murine thymus." in: Molecular cancer research : MCR, Vol. 3, Issue 10, pp. 553-61, (2005) (PubMed).
抗原

DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

别名

Dnmt3a

背景

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.

分子量

101858

基因ID

1788

NCBI登录号

NP_072046, NP_715640, NP_783328, NP_783329

UniProt

Q9Y6K1