GFP Tag 抗体
(2 references)Quick Overview for GFP Tag 抗体 (ABIN387749)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
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免疫原
- Purified His-tagged GFP protein was used to produced this monoclonal antibody.
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亚型
- IgG
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anti-GFP Tag antibody
适用: 人 WB, IF 宿主: 小鼠 Monoclonal 168AT1211 unconjugated
anti-GFP Tag antibodyWB 宿主: 兔 Polyclonal unconjugated
anti-GFP Tag (N-Term) antibody适用: Aequorea victoria WB, ELISA, IHC 宿主: 小鼠 Monoclonal LGB-1 unconjugated
anti-GFP Tag antibody适用: Aequorea victoria ELISA, IF, ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB 宿主: 小鼠 Polyclonal unconjugated
anti-Green Fluorescent Protein (GFP) antibodyGFP 适用: Aequorea victoria WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB: 1:100~500. WB: 1:100~500
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Mouse monoclonal antibody supplied in crude ascites with 0.09 % (W/V) sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
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有效期
- 6 months
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: "Functional comparison of RNA silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus." in: Virus research, Vol. 203, pp. 10-9, (2015) (PubMed).
: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, (2011) (PubMed).
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: "Functional comparison of RNA silencing suppressor between the p5 protein of rice grassy stunt virus and the p3 protein of rice stripe virus." in: Virus research, Vol. 203, pp. 10-9, (2015) (PubMed).
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- GFP Tag
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物质类
- Tag
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背景
- Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
抗原
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