Sca-1/Ly-6A/E 抗体 (APC)
Quick Overview for Sca-1/Ly-6A/E 抗体 (APC) (ABIN375409)
抗原
See all Sca-1/Ly-6A/E (Ly6a) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Mouse Ly-6A/E (Ly-6A.2 & Ly-6E.1), Mr 18 kDa
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产品特性
- Rat Anti-Mouse Ly-6A/E-APC
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纯化方法
- Purified
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免疫原
- IL-2 dependent mouse (C57BL/6) cytotoxic T cell line CTL-L
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亚型
- IgG2a
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应用备注
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- Applications: FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , WB - Reported in literature , Costim - Reported in literature
- Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE, APC, SPRD, PE/CY5.5, PACBLU, and AF647 conjugates 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
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说明
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In vitro lymphocyte activation
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样本量
- 1 mL
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限制
- 仅限研究用
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浓度
- 0.1 mg/mL
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缓冲液
- 0.1 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
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Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
储存条件
- 4 °C
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储存方法
- Store at 2-8°C
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The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice." in: Journal of cellular and molecular medicine, (2014) (PubMed).
: "Irradiated stem cells and ageing of the haematopoietic system." in: Radiation and environmental biophysics, (2012) (PubMed).
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The peripheral chimerism of bone marrow-derived stem cells after transplantation: regeneration of gastrointestinal tissues in lethally irradiated mice." in: Journal of cellular and molecular medicine, (2014) (PubMed).
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- Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))
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别名
- Ly-6A/E
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背景
- Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E + resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E + lymphocytes. The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.
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途径
- Sensory Perception of Sound, Activated T Cell Proliferation
抗原
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