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EGR2 抗体 (Internal Region)

The 山羊 多克隆 anti-EGR2 antibody is suitable to detect EGR2 in samples from 人, 小鼠, 大鼠 和 犬. It has been validated for WB, IHC (p) 和 EIA.
产品编号 ABIN375194
发货至: 中国
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Quick Overview for EGR2 抗体 (Internal Region) (ABIN375194)

抗原

See all EGR2 抗体
EGR2 (Early Growth Response 2 (EGR2))

适用

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人, 小鼠, 大鼠, 犬

宿主

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山羊

克隆类型

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多克隆

标记

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This EGR2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    Internal Region

    序列

    HGTAGPDRKP FPC

    特异性

    This antibody recognizes EGR2

    交叉反应 (详细)

    Species reactivity (expected):Mouse, Rat and Canine.
    Species reactivity (tested):Human.

    纯化方法

    Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence from the internal region of the protein sequence according to NP_000390.2. Genename: EGR2
  • 应用备注

    Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in lysates of cell line HepG2(calculated MW of 50.5 kDa according to NP_000390.2). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Colon shows nuclearstaining of absorptive cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    EGR2 (Early Growth Response 2 (EGR2))

    别名

    EGR2

    背景

    ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.Synonyms: AT591, EGR-2, Early growth response protein 2, KROX20, Krox 20, Zinc finger protein Krox-20

    基因ID

    1959

    NCBI登录号

    NP_000390

    UniProt

    P11161
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