EGR2 抗体 (Internal Region)
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Quick Overview for EGR2 抗体 (Internal Region) (ABIN375194)
抗原
See all EGR2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- HGTAGPDRKP FPC
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特异性
- This antibody recognizes EGR2
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat and Canine.
Species reactivity (tested):Human. -
纯化方法
- Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_000390.2. Genename: EGR2
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应用备注
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Peptide ELISA: Detection Limit: 1/64000. Western blot: 0.03-0.1 μg/mL. Approx 55 kDa band observed in lysates of cell line HepG2(calculated MW of 50.5 kDa according to NP_000390.2). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Colon shows nuclearstaining of absorptive cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- EGR2 (Early Growth Response 2 (EGR2))
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别名
- EGR2
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背景
- ERG2 is a sequence-specific transcription factor, that binds to two specific DNA sites located in the promoter region of HOXA4. Defects in EGR2 are a cause of congenital hypomyelination neuropathy (CHN), which can be inherited in either an autosomal dominant or recessive manner. Recessive CHN can present as Charcot-Marie-Tooth disease type 4E, type 1D, or Dejerine-Sottas syndrome (DSS), a group of neuropathies associated with decreased nerve conduction velocities.Synonyms: AT591, EGR-2, Early growth response protein 2, KROX20, Krox 20, Zinc finger protein Krox-20
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基因ID
- 1959
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NCBI登录号
- NP_000390
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UniProt
- P11161
抗原
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