ERP29 抗体 (C-Term)
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Quick Overview for ERP29 抗体 (C-Term) (ABIN375036)
抗原
See all ERP29 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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特异性
- This antibody reacts to ERP29.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat, Canine, Bovine, Porcine.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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免疫原
- Peptide with sequence C-EKNKMSDGKKEELQ, from the C-Terminus of the protein sequence
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应用备注
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Peptide ELISA: 1/2000. Western Blot: 0.1 - 0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0,5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ERP29 (Endoplasmic Reticulum Protein 29 (ERP29))
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别名
- ERP29
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背景
- Proper protein folding and post-translational modifications are essential for secretory protein export out of the endoplasmic reticulum. This task is accomplished by chaperone proteins such as protein disulfide isomerase (PDI), GRP94, and BiP. A recently characterized protein, designated ERp29, is closely related to these chaperone proteins and appears to be upregulated during ER stress conditions. ERp29 is a soluble 259-residue protein that is localized to the lumen of the endoplasmic reticulum in all mammalian cells. Research has shown that there are two primary domains within ERp29. The first is the C-terminal region that is a novel, all helical, fold that is most likely involved with ERp29 retention to the ER. The second is the N-terminal region that resembles that of PDI's thioredoxin module. The protein shows sequence similarity to the protein disulfide isomerase family. However, it lacks the thioredoxin motif characteristic of this family, suggesting that this protein does not function as a disulfide isomerase. The protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER.Synonyms: C12orf8, ERp28, ERp31, Endoplasmic reticulum protein ERp29
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基因ID
- 10961
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NCBI登录号
- NP_006808
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UniProt
- P30040
抗原
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