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EGR4 抗体 (Internal Region)

This 山羊 多克隆 antibody specifically detects EGR4 in WB. It exhibits reactivity toward 人.
产品编号 ABIN374881
发货至: 中国
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Quick Overview for EGR4 抗体 (Internal Region) (ABIN374881)

抗原

See all EGR4 抗体
EGR4 (Early Growth Response 4 (EGR4))

适用

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宿主

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山羊

克隆类型

  • 16
多克隆

标记

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This EGR4 antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

    • 2
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    Internal Region

    序列

    NCGSQGDYQA APE

    交叉反应 (详细)

    Species reactivity (tested):Human

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from the internal region of the protein sequence according to NP_001956.2
  • 应用备注

    Peptide ELISA: limit dilution 1: 4000. Western blot: 0.3-1 μg/mL. Approx 50 kDa band observed in Human Brain (Cerebellum andFrontal Cortex) lysates (calculated MW of 50.0 kDa according to NP_001956.2).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • 抗原

    EGR4 (Early Growth Response 4 (EGR4))

    别名

    EGR4

    背景

    EGR4(Early Growth Response protein 4) is a zinc-finger transcription factor that activates the transcription of genes whose products are required for mitogenesis and differentiation. EGR4 is closely related to nerve growth factor-induced clone A (NGFI-A or EGR1), EGR2, and EGR3. These four early response (immediate early) proteins all contain very similar zinc-finger DNA binding domains and five highly homologous subdomains. EGR4 functionally cooperate with NFAT proteins and induce expression of IL2 and TNFalpha. Early growth response proteins (EGR) and nuclear factors of activated T cells (NFAT) form heterodimers and regulate proinflammatory cytokine gene expression.Synonyms: AT133, EGR-4, Early growth response protein 4, NGFI-C, NGFIC, PAT133

    基因ID

    9606

    UniProt

    Q05215
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