ADH5 抗体 (Internal Region)
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北京 101111
Quick Overview for ADH5 抗体 (Internal Region) (ABIN374869)
抗原
See all ADH5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-KKIKVDEFVT HN
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特异性
- This antibody detects Alcohol Dehydrogenase 5 (ADH5).
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交叉反应 （详细）
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Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region of the protein sequence according to NP_000662.3 Genename: ADH5
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anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 212-239) antibody
ADH5 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB22372 unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 1-374) antibodyADH5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 1-374) antibodyADH5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2D11 unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 14-130) antibodyADH5 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 212-374) antibodyADH5 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 1-374) antibodyADH5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 1-374) antibodyADH5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 12F7 unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (C-Term) antibodyADH5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) (AA 82-165) antibodyADH5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5) antibodyADH5 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB4695 unconjugated Recombinant Antibody
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应用备注
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Peptide ELISA: antibody detection limit dilution 1: 1000. Western blot: 1-3 μg/mL. Customer's experiments gave a band at approx 38 kDa in MouseLymphocyte lysates (calculated MW of 39.8 kDa according to Human NP_000662.3 and39.8 kDa according to Mouse NP_031436.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))
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别名
- Alcohol Dehydrogenase 5 (ADH5)
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背景
- The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.Synonyms: Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase
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分子量
- 39.7 kDa according to NP_000662.3.
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基因ID
- 128, 9606
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UniProt
- P11766
抗原
-
