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TBC1D4 抗体 (Internal Region)

The 山羊 多克隆 anti-TBC1D4 antibody is suitable to detect TBC1D4 in samples from 人 和 犬. It has been validated for WB 和 IHC (p).
产品编号 ABIN374867
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for TBC1D4 抗体 (Internal Region) (ABIN374867)

抗原

See all TBC1D4 抗体
TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

适用

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人, 犬

宿主

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山羊

克隆类型

  • 67
  • 17
多克隆

标记

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This TBC1D4 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

    • 27
    • 15
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    Internal Region

    序列

    C-DDPEKIEERK KSK

    特异性

    This antibody detects internal TBC1D4 / AS160.

    交叉反应 (详细)

    Species reactivity (expected):DogSpecies reactivity (tested):Human

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from the internal region of the protein sequence according to NP_055647.2 Genename: TBC1D4
  • 应用备注

    Peptide ELISA: antibody detection limit dilution 1: 128000. Western Blot: Approx 150 kDa band observed in lysates of cell lines Daudi and MOLT4(calculated MW of 140 kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry on paraffin sections: 3-5 μg/mL. In Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
  • 抗原

    TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))

    别名

    TBC1D4 / AS160

    背景

    TBC1D4 is a Rab GTPase activating protein (GAP) for Rab family protein(s) (referenced from swissprot).Synonyms: Akt substrate of 160 kDa, KIAA0603, TBC1 domain family member 4

    基因ID

    9882, 9606

    UniProt

    O60343
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