TBC1D4 抗体 (Internal Region)
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Quick Overview for TBC1D4 抗体 (Internal Region) (ABIN374867)
抗原
See all TBC1D4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-DDPEKIEERK KSK
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特异性
- This antibody detects internal TBC1D4 / AS160.
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交叉反应 (详细)
- Species reactivity (expected):DogSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from the internal region of the protein sequence according to NP_055647.2 Genename: TBC1D4
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应用备注
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Peptide ELISA: antibody detection limit dilution 1: 128000. Western Blot: Approx 150 kDa band observed in lysates of cell lines Daudi and MOLT4(calculated MW of 140 kDa according to NP_055647.2). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry on paraffin sections: 3-5 μg/mL. In Human Skeletal Muscle shows striated pattern staining of majority of muscular fibres.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- TBC1D4 (TBC1 Domain Family, Member 4 (TBC1D4))
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别名
- TBC1D4 / AS160
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背景
- TBC1D4 is a Rab GTPase activating protein (GAP) for Rab family protein(s) (referenced from swissprot).Synonyms: Akt substrate of 160 kDa, KIAA0603, TBC1 domain family member 4
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基因ID
- 9882, 9606
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UniProt
- O60343
抗原
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