ABHD5 抗体 (Internal Region)
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Quick Overview for ABHD5 抗体 (Internal Region) (ABIN374842)
抗原
See all ABHD5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody recognizes CGI58 / ABHD5. AP16647PU-N is not expected to cross-react with ABHD4.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.
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免疫原
- Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence Genename: ABHD5
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anti-Abhydrolase Domain Containing 5 (ABHD5) (AA 1-349) antibody
ABHD5 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) (Internal Region) antibodyVerified ABHD5 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) (AA 240-342) antibodyABHD5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) (AA 240-342) antibodyABHD5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4B12 unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) antibodyKD Validated ABHD5 适用: 人 WB, FACS 宿主: 兔 Monoclonal 23GB3260 unconjugated Recombinant Antibody
anti-Abhydrolase Domain Containing 5 (ABHD5) (C-Term) antibodyABHD5 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 兔, 豚鼠, Pig, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) antibodyABHD5 适用: 人 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) (AA 281-349) antibodyABHD5 适用: 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) (AA 1-350) antibodyABHD5 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Abhydrolase Domain Containing 5 (ABHD5) (Internal Region) antibodyABHD5 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: ABHD5 Antibody detection limit dilution: 1/16000. Western Blot: 0.2-0.6 μg/mL. Approx 40 kDa band observed in lysates of cell lines NIH3T3and A431 calculated MW of 39.0 kDa according to NP_057090.2). Additional faint bands of20 kDa and 10 kDa were observed. These bands were successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))
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别名
- ABHD5
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背景
- ABHD5 belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. ABHD5 is widely expressed in various tissues, including skin, lymphocytes, liver, skeletal muscle and brain.Synonyms: Abhydrolase domain-containing protein 5, CGI-58, NCIE2
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基因ID
- 51099, 9606
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UniProt
- Q8WTS1
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途径
- Lipid Metabolism
抗原
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