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Liver Arginase 抗体 (Arg1, C-Term)

The 山羊 多克隆 anti-Liver Arginase antibody (ABIN374811) specifically detects Liver Arginase in WB. The antibody is reactive with 人 和 犬 samples.
产品编号 ABIN374811
发货至: 中国
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Quick Overview for Liver Arginase 抗体 (Arg1, C-Term) (ABIN374811)

抗原

See all Liver Arginase (ARG1) 抗体
Liver Arginase (ARG1) (Arginase, Liver (ARG1))

适用

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人, 犬

宿主

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山羊

克隆类型

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  • 1
多克隆

标记

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This Liver Arginase antibody is un-conjugated

应用范围

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Western Blotting (WB)
  • 抗原表位

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    Arg1, C-Term

    序列

    C-REGNHKPIDY LNPPK

    特异性

    This antibody detects Arginase-1.

    交叉反应 (详细)

    Species reactivity (expected):Canine (Dog).
    Species reactivity (tested):Human.

    纯化方法

    Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

    免疫原

    Peptide from the C Terminus of the protein sequence according to NP_000036.2 Genename: ARG1
  • 应用备注

    Peptide ELISA: antibody detection limit dilution 1: 2000. Western blot: 0.03-0.1 μg/mL. Approx 35 kDa band observed in Human Liver lysates(calculated MW of 34.5 kDa according to NP_000036.2. Additional bands of unknown identity were also consistently observed at 19-25 kDa. Thiesebands were successfully blocked by incubation with the immunising peptide. We wouldappreciate any feedback from people in the field - have any such results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    Liver Arginase (ARG1) (Arginase, Liver (ARG1))

    别名

    Arginase-1

    背景

    Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.Synonyms: ARG1, Liver-type arginase, Type I arginase

    基因ID

    383, 9606

    UniProt

    P05089

    途径

    Cellular Response to Molecule of Bacterial Origin
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