Glucose-6-Phosphate Dehydrogenase 抗体 (Internal Region)
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Quick Overview for Glucose-6-Phosphate Dehydrogenase 抗体 (Internal Region) (ABIN374790)
抗原
See all Glucose-6-Phosphate Dehydrogenase (G6PD) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 308-320, Internal Region
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序列
- C-STNSDDVRDE KVK
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特异性
- This antibody is expected to recognise both reported isoforms (NP_000393.4 and NP_001035810.1).
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat and Dog (Canine).
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.
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免疫原
- Synthetic Peptide from the internal region (aa 308-320) of Human G6PD. Genename: G6PD
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应用备注
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Peptide ELISA: Limit dilution: 1/32000. Western Blot: Use at 0.03-0.1 μg/mL. Detects a double band of approx. 55-60 kDa band in HumanTestis, Thyroid and Tonsil lysates. Immunohistochemistry: 2-4 μg/mL. In paraffin embedded Human Testis shows strongcytoplasm staining in spermatogonia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month (in aliquots) at -20 °C for longer.
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- Glucose-6-Phosphate Dehydrogenase (G6PD)
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别名
- G6PD
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背景
- Glucose 6 Phosphate Dehydrogenase (G6PD) produces pentose sugars for nucleic acid synthesis and is the main producer of NADPH reducing power. Catalytic activity: D glucose 6 phosphate + NADP(+) = D glucono 1,5 lactone 6 phosphate + NADPH. Defects in G6PD are the cause of chronic non spherocytic haemolytic anemia (CNSHA). G6PD deficiency is the most common human enzyme deficiency, one benefit of having G6PD deficiency is that it confers a resistance to malaria.Synonyms: Glucose-6-P-Dehydrogenase, Glucose-6-phosphate 1-dehydrogenase
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基因ID
- 2539, 9606
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UniProt
- P11413
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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