SV2A 抗体 (Internal Region)
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Quick Overview for SV2A 抗体 (Internal Region) (ABIN374729)
抗原
See all SV2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody recognizes SV2A.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Human, Canine.
Species reactivity (tested):Rat. -
纯化方法
- Affinity chromatography
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免疫原
- Peptide with sequence C-TVELYPSDKRTTA, from the internal region of the protein sequence
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应用备注
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Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in rat brain lysates (calculatedMW of 82.0 kDa according to NP_476558.1 (rat)). An additonal band of unknown identitywas also consistently observed at 20 kDa. This band was successfully blocked byincubation with the immunising peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % Sodium Azide, pH 7.3 with 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))
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别名
- SV2A
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背景
- SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.Synonyms: KIAA0736, Synaptic vesicle glycoprotein 2A
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基因ID
- 9900
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NCBI登录号
- NP_055664
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UniProt
- Q7L0J3
抗原
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