Glutathione Peroxidase 1 抗体 (Internal Region)
Quick Overview for Glutathione Peroxidase 1 抗体 (Internal Region) (ABIN374697)
抗原
See all Glutathione Peroxidase 1 (GPX1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-REALPAPSDD ATA
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特异性
- This antibody is expected to recognise Human Glutathione Peroxidase 1 Isoform 1 only (NP_000572.2).
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交叉反应 （详细）
- Species reactivity (tested):Human.
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纯化方法
- Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Syntehtic Peptide from the internal region of Human GPX1 (NP_000572.2). Genename: GPX1
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anti-Glutathione Peroxidase 1 (GPX1) (AA 50-203) antibody
GPX1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 164-193), (C-Term) antibodyGPX1 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB22987 unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (Internal Region) antibodyVerified GPX1 适用: 人, Pig WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 50-203) antibodyGPX1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 116-146), (Middle Region) antibodyGPX1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 48-201) antibodyGPX1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 127-193) antibodyGPX1 适用: 人 WB, IHC, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 169-199), (C-Term) antibodyGPX1 适用: 人, 小鼠 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 51-150) antibodyGPX1 适用: 人, 小鼠, Pig, 绵羊 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Glutathione Peroxidase 1 (GPX1) (AA 48-201) antibodyGPX1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Detection Limit: 1/32000. Western blot: 1-3 μg/mL. Approx 22 kDa band observed in Human Liver and Kidney lysates(calculated MW of 22.2 kDa according to NP_000572.2). Immunohistochemistry: 3.5 μg/mL. In paraffin embedded Human Cerebral Cortex shows staining of microglia.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Glutathione Peroxidase 1 (GPX1)
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别名
- Glutathione Peroxidase 1 / GPX1
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背景
- Glutathione peroxidases(GPxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. This protein is one of the most important antioxidant enzymes in humans. This protein has been reported to protect cultured breast cancer cells from CD95-induced apoptosis and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. There are several isoforms which differ in their primary structure and localization. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis specific secretory GPx(eGPxor GPx5)isoforms, possess selenocysteine at the active site. One allele of the Glutathione Peroxidase 1 gene is significantly associated with an increased breast cancer risk.Synonyms: Cellular glutathione peroxidase, GSHPx-1
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基因ID
- 2876
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NCBI登录号
- NP_000572
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UniProt
- P07203
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途径
- Thyroid Hormone Synthesis, Sensory Perception of Sound, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis, Negative Regulation of intrinsic apoptotic Signaling, SARS-CoV-2 Protein Interactome
抗原
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