AGO1 抗体 (Internal Region)
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Quick Overview for AGO1 抗体 (Internal Region) (ABIN374655)
抗原
See all AGO1 (EIF2C1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- This antibody recognizes Protein eIF2C1 /AGO1. This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide with sequence C-KNASYNLDPYIQEF, from the internal region of the protein sequence according to NP_036331.1.
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anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-180) antibody
EIF2C1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) antibodyEIF2C1 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 251-400) antibodyEIF2C1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (Internal Region) antibodyVerified EIF2C1 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56) antibodyEIF2C1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠, 犬, 猴, Cow, 马, 兔, 豚鼠, Pig, 非洲爪蟾, Bat WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 27-56), (N-Term) antibodyEIF2C1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB18965 unconjugated
anti-Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (N-Term) antibodyEIF2C1 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 斑马鱼, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Detection Limit: 1/16000. Western Blot: 0.3-1 μg/mL. In transfected HEK293 transiently expressing Human EIF2C1bands of approx.110 kDa and 30 kDa are observed. The 110 kDa band is not observed in thenon-transfected HEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
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别名
- EIF2C1 / AGO1
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背景
- Ago1 belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as cell cycle control protein through it's regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.Synonyms: Argonaute-1, Eukaryotic translation initiation factor 2C1, RNA-binding protein Q99, eIF-2C1, hAgo-1
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分子量
- 97.2kDa according to NP_036331.1
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基因ID
- 26523
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NCBI登录号
- NP_036331
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UniProt
- Q9UL18
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途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
抗原
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