PPIA 抗体 (Internal Region)
Quick Overview for PPIA 抗体 (Internal Region) (ABIN374639)
抗原
See all PPIA 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- C-ERFGSRNGKT SKK
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特异性
- This antibody is expected to recognise Cyclophilin A. This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_066953.1. Genename: PPIA
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anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-165) antibody
PPIA 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-164) antibodyPPIA 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-164) antibodyPPIA 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 1-165) antibodyPPIA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F4-1B5 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 116-165) antibodyPPIA 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, FACS, IF, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (C-Term) antibodyKO Validated PPIA 适用: 人 WB, ICC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-165) antibodyPPIA 适用: 人 WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal C10 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 2-165) antibodyPPIA 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 8-37), (N-Term) antibodyPPIA 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB20884 unconjugated
anti-Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA) (AA 101-165) antibodyPPIA 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 μg/mL. Approx 18 kDa band observed in Human Lymph Node lysates(calculated MW 18.8 kDa according to NP_066953.1).
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))
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别名
- Cyclophilin A
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背景
- Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.Synonyms: CYPA, Cyclophilin-A, Cyclosporin A-binding protein, EC=5.2.1.8, PPIA, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Rotamase A
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基因ID
- 5478
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NCBI登录号
- NP_066953
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UniProt
- P62937
抗原
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