PEPCK 抗体 (Internal Region)
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北京 101111
Quick Overview for PEPCK 抗体 (Internal Region) (ABIN374610)
抗原
See all PEPCK 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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序列
- KPWKPGDKEP CAH
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特异性
- This antibody recognizes PCK2. This antibody is expected to recognise isoform 1 only, no crossreactivity with PCK1.
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交叉反应 （详细）
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Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Human, Mouse, Rat, Guinea Pig. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_004554.2. Genename: PCK2
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anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (Internal Region) antibody
Verified PEPCK 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 44-175) antibodyPEPCK 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3D3D9 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibodyPEPCK 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibodyPEPCK 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, IF, ICC, FACS 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibodyPEPCK 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 24-54), (N-Term) antibodyPEPCK 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB3640 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) antibodyPEPCK 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 33-198) antibodyPEPCK 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-640) antibodyPEPCK 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C9 unconjugated
anti-phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 1-280) antibodyPEPCK 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit Dilution: 1/1000. Western blot: 0.1-0.3 μg/mL. Approx 70 kDa band observed in Human Liver lysates and inlysates of cell line HepG2 (calculated MW of 70.0 kDa according to NP_004554.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).
: "Light sensing in Aspergillus fumigatus highlights the case for establishing new models for fungal photobiology." in: mBio, Vol. 4, Issue 3, pp. e00260-13, (2013) (PubMed).
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- PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
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别名
- PCK2
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背景
- PCK2 is a a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.Synonyms: EC=4.1.1.32, PCK-2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase
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基因ID
- 5106
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NCBI登录号
- NP_001018083
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UniProt
- Q16822
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途径
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
抗原
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