RAE1 抗体 (C-Term)
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Quick Overview for RAE1 抗体 (C-Term) (ABIN374586)
抗原
See all RAE1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-NAAEELKPRN KK
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特异性
- This antibody recognizes RAE1.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence from the C Terminus of Human RAE1 Genename: RAE1
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应用备注
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Peptide ELISA: Detection Limit: 1/64000. Western Blot: 0.03-0.1 μg/mL. Detects a band of Approx 38-40 kDa in lysates of HumanHepatocarcinoma cell line HepG2.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- RAE1 (RAE1 RNA Export 1 Homolog (S. Pombe) (RAE1))
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别名
- RAE1
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背景
- Mutations in Rae1 and Gle2 (Saccharomyces cerevisae homolog) genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Depletion of Rae1 from extracts or cells severely inhibits mitotic spindle assembly. Alternatively spliced transcript variants encoding the same protein have been found for this gene.Synonyms: MRNP41, mRNA export factor, mRNA-associated protein mrnp 41
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基因ID
- 8480, 9606
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UniProt
- P78406
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途径
- SARS-CoV-2 Protein Interactome
抗原
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