ADH1A 抗体 (N-Term)
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Quick Overview for ADH1A 抗体 (N-Term) (ABIN374506)
抗原
See all ADH1A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- This antibody is expected to recognize the alpha (ADH1A, NP_ 000658.1), the beta (ADH1B, NP_000659.2) and gamma (ADH1C, NP_000660.1) polypeptide variants of Human alcohol dehydrogenase.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence STAGKVMKCKA, from the N Terminus of the protein sequence according to NP_000658.1, NP_000659.2, NP_000660.1.
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应用备注
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Peptide ELISA: 1/16000. Western Blot: 0.3-1.5 μg/mL. Approx 38 kDa band observed in Human Liver lysates(calculated MW of 39.8 kDa according to NP_000658.1, NP_000659.2 and 000660.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as presrevative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ADH1A (Alcohol Dehydrogenase 1A (Class I), alpha Polypeptide (ADH1A))
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别名
- Alcohol Dehydrogenase 1A (ADH1)
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背景
- ADH (Alcohol dehydrogenase) consists of a dimer of identical or nonidentical chains of three types, alpha, beta and gamma. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. There are 7 different ADH's isozymes in human: three belongs to class I: alpha, beta, and gamma, one to class II: pi, one to class III: chi, one to class IV: ADH7 and one to class V: ADH6.Synonyms: ADH1A, Alcohol dehydrogenase subunit alpha
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基因ID
- 124, 9606
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UniProt
- P07327
抗原
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