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FHL1 抗体 (Internal Region)

The 山羊 多克隆 anti-FHL1 antibody is suitable to detect FHL1 in samples from 人, 小鼠, 大鼠, 犬, Cow 和 Pig. It has been validated for WB, IHC (p) 和 EIA.
产品编号 ABIN374471
发货至: 中国
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Quick Overview for FHL1 抗体 (Internal Region) (ABIN374471)

抗原

See all FHL1 抗体
FHL1 (Four and A Half LIM Domains 1 (FHL1))

适用

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人, 小鼠, 大鼠, 犬, Cow, Pig

宿主

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山羊

克隆类型

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多克隆

标记

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This FHL1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
  • 抗原表位

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    Internal Region

    特异性

    This antibody is expected to recognise isoforms 2 (NP_001440.1) and 5 (NP_001153171.1). Since June 2009 the second N residue in the immunizing peptide is no longer representative as it has been substituted by Q at position 284 of NP_001440.2. Variants NP_001440.2, NP_001153176.1 and NP_001153172.1 represent identical protein.

    交叉反应 (详细)

    Species reactivity (expected):Rat, Canine, Porcine and Bovine.
    Species reactivity (tested):Human, Mouse.

    纯化方法

    Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

    免疫原

    Peptide with sequence CNKRFVFHNEQVY, from the internal region of the protein sequence according to NP_001440.1, NP_001153171.1. Genename: FHL1
  • 应用备注

    Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.05-0.3 μg/mL. Detects a band of Approx 30 kDa in Human Muscle lysates(calculated MW of 31.0 kDa according to NP_001440). Immunohistochemistry: 2-2 μg/mL. In paraffin embedded Human Testis shows fibrousstaining in the cytoplasm of developing sperm cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • 抗原

    FHL1 (Four and A Half LIM Domains 1 (FHL1))

    别名

    FHL1

    背景

    FHL1 (SLIM1) is a LIM domain protein transcript that is preferentially expressed in postnatal skeletal muscle but little is known about its function in this tissue. LIM proteins, (named for 'LIN11, ISL1 and MEC3') are defined by the possession of a highly conserved double zinc finger motif called the LIM domain. FHL1 may have an involvement in muscle development or hypertrophy. Isoform 2 binds to RBP J and plays a negative regulatory role in the RBP J mediated transcription in mammalian systems.Synonyms: FHL-1, FHL1, Four and a half LIM domains protein 1, SLIM, SLIM-1, SLIM1, Skeletal Muscle LIM-protein 1

    基因ID

    2273

    NCBI登录号

    NP_001153171

    UniProt

    Q13642
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