ACTR1B 抗体 (C-Term)
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北京 101111
Quick Overview for ACTR1B 抗体 (C-Term) (ABIN374429)
抗原
See all ACTR1B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-YEEDGSRAIH RKT
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特异性
- This antibody recognizes ARP1 homolog B.
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
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免疫原
- Peptide from the C Terminus of the protein sequence according to NP_005726. Genename: ACTR1B
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应用备注
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Peptide ELISA: 1/128000. Western Blot: 0.5-2 μg/mL. Approx 45 kDa band observed in human heart and brain lysates(predicted MW of 45 kDa according to NP_005726). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Uterus shows fibrillar staining of cytoplasm in endometrialcells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ACTR1B (ARP1 Actin-Related Protein 1 Homolog B, Centractin beta (ACTR1B))
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别名
- Centractin beta
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背景
- In addition to conventional actins, there are several actin-related proteins. The ACTR1B protein and the ACTR1A protein are of equal length, and they share 90 % amino acid identity and 96 % amino acid similarity. ACTR1B is expressed in the cytosol as part of the dynactin complex. The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dynein mediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1B appears to be involved in dynein-driven vesicle movement.Synonyms: ACTR1B, ARP1B, Actin-related protein 1B, CTRN2
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基因ID
- 10120
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NCBI登录号
- NP_005726
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UniProt
- P42025
抗原
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