KPNA1 抗体 (N-Term)
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Quick Overview for KPNA1 抗体 (N-Term) (ABIN374391)
抗原
See all KPNA1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- TTPGKENFRL KSY-C
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特异性
- This antibody detects Importin subunit alpha-1 at N-term.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
Species reactivity (tested):Human -
纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1
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anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-300) antibody
KPNA1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) antibodyKPNA1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A4-1B5 unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) antibodyKPNA1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) antibodyKPNA1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) antibodyKD Validated KPNA1 适用: 人 WB, FACS 宿主: 小鼠 Monoclonal 25GB700 unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-538) antibodyKPNA1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 239-506) antibodyKPNA1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 8-538) antibodyKPNA1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (N-Term) antibodyKPNA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Karyopherin alpha 1 (Importin alpha 5) (KPNA1) (AA 1-539) antibodyKPNA1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2A4-1B5 unconjugated
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应用备注
- Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))
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别名
- KPNA1 / Importin alpha-1
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背景
- Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta
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基因ID
- 3836
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NCBI登录号
- NP_002255
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UniProt
- P52294
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途径
- M Phase, Protein targeting to Nucleus
抗原
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