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KPNA1 抗体 (N-Term)

This anti-KPNA1 antibody is a 山羊 多克隆 antibody detecting KPNA1 in WB. Suitable for 人, 小鼠, 大鼠, Cow, 犬 和 Pig.
产品编号 ABIN374391
发货至: 中国

Quick Overview for KPNA1 抗体 (N-Term) (ABIN374391)

抗原

See all KPNA1 抗体
KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

适用

  • 38
  • 18
  • 17
  • 4
  • 4
  • 4
  • 4
  • 4
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  • 2
  • 2
  • 2
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  • 1
人, 小鼠, 大鼠, Cow, 犬, Pig

宿主

  • 29
  • 8
  • 1
山羊

克隆类型

  • 33
  • 5
多克隆

标记

  • 27
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
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  • 1
This KPNA1 antibody is un-conjugated

应用范围

  • 27
  • 15
  • 9
  • 7
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB)
  • 抗原表位

    • 9
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    N-Term

    序列

    TTPGKENFRL KSY-C

    特异性

    This antibody detects Importin subunit alpha-1 at N-term.

    交叉反应 (详细)

    Species reactivity (expected):Mouse, Rat, Dog Pig, Cow.
    Species reactivity (tested):Human

    纯化方法

    Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

    免疫原

    Peptide from N Terminus of the protein sequence according to NP_002255.3 Genename: KPNA1
  • 应用备注

    Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 60 kDa band observed in lysates of cell lines HEK293, HeLaand HEPG2 (calculated MW of 60.0 kDa according to NP_002255.1).

    限制

    仅限研究用
  • 浓度

    0.5 mg/mL

    缓冲液

    Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
  • 抗原

    KPNA1 (Karyopherin alpha 1 (Importin alpha 5) (KPNA1))

    别名

    KPNA1 / Importin alpha-1

    背景

    Protein transfer into and out of the nucleus occurs through the nuclear pore complex (NPCs). Karyopherin, a cytosolic, heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex. SRP1 (nucleoprotein interactor 1, NPI 1) is the alpha subunit of karyopherin, which acts as the receptor for the targeted protein's nuclear localization signal (Moroianu J. Crit 1997). Both subunits of karyopherin are required for protein docking at the NPC, the alpha subunit binds the substrate and the beta subunit acts as an adaptor (Moroianu J, et al. 1995). Two other proteins, p10 and the GTPase Ran, are also required for the translocation of docked proteins to the nucleus. The karyopherin dimer dissociates during import, a process mediated by RanGTP, which binds to karyopherin ß and promotes the release of both subunits from the docking site (Moroianu J. Crit 1997). The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC (Moroianu J. Crit 1997)Synonyms: IPOA5, Karyopherin alpha 1, NPI-1, Nucleoprotein interactor 1, RAG cohort protein 2, RCH2, SRP1, SRP1-beta

    基因ID

    3836

    NCBI登录号

    NP_002255

    UniProt

    P52294

    途径

    M Phase, Protein targeting to Nucleus
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