GRAF 抗体 (C-Term)
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Quick Overview for GRAF 抗体 (C-Term) (ABIN374371)
抗原
See all GRAF (ARHGAP26) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-KTGLIPENYV EFL
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特异性
- This antibody detects ARHGAP26/OPHN1L at C-term. It is expected to recognise both reported isoforms.
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交叉反应 (详细)
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Species reactivity (expected):Mouse, Rat, Canine (Dog) and Bovine (Cow).
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C-Terminus of the protein sequence according to NP_055886.1, NP_001129080.1. Genename: ARHGAP26
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应用备注
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Peptide ELISA: Limit dilution 1/64000. Western blot: 0.3-1 μg/mL. Approx 90 kDa band observed in Human Heart lysates(calculated MW of 92.0 kDa according to NP_055886.1). An additional strong band ofunknown identity was also consistently observed at 10 kDa. This band was successfullyblocked by incubation with the immunising peptide. We would appreciate any feedbackfrom people in the field - have any results been reported with other antibodies/lysates?Have any further splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 with 0.02 % Sodium Azide and 0.5 % BSA
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
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- GRAF (ARHGAP26) (rho GTPase Activating Protein 26 (ARHGAP26))
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别名
- ARHGAP26 / OPHN1L
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背景
- Synonyms: GRAF, GTPase regulator associated with focal adhesion kinase, KIAA0621, Oligophrenin-1-like protein, Rho GTPase-activating protein 26, Rho-type GTPase-activating protein 26
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基因ID
- 23092
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NCBI登录号
- NP_055886
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UniProt
- Q9UNA1
抗原
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