ARHGEF4 抗体 (N-Term)
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Quick Overview for ARHGEF4 抗体 (N-Term) (ABIN374361)
抗原
See all ARHGEF4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- PWEEPAGEKP SCS
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特异性
- This antibody detects ARHGEF4 at N-term.
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交叉反应 (详细)
- Species reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from N Terminus of the protein sequence according to NP_056135.2, NP_127462.1. Genename: ARHGEF4
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 2-4 μg/mL. Approx 80 kDa band observed in Human Brain lysates and intransfected HEK293 transiently expressing ARHGEF4 (calculated MW of 79.0 kDa accordingto NP_056135.2 and 76.0 kDa according to 127462.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- ARHGEF4 (rho Guanine Nucleotide Exchange Factor (GEF) 4 (ARHGEF4))
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别名
- ARHGEF4
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背景
- ARHGEF4 may form complexes with G proteins and stimulate Rho-dependent signals, and is similar to rat collybistin protein. Rho GTPases play a fundamental role in numerous cellular processes, which are initiated by extracellular stimuli that work through G protein coupled receptors. Alternative splicing of this gene generates two transcript variants which encode different isoforms.Synonyms: APC-stimulated guanine nucleotide exchange factor, ASEF, KIAA1112, Rho guanine nucleotide exchange factor 4
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基因ID
- 50649
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NCBI登录号
- NP_056135
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UniProt
- Q9NR80
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途径
- Neurotrophin Signaling Pathway
抗原
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