FOXA1 抗体 (C-Term)
Our Local Distributor
北京 101111
Quick Overview for FOXA1 抗体 (C-Term) (ABIN374334)
抗原
See all FOXA1 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- C-Term
-
序列
- C-GVYSRPVLNT S
-
特异性
- This antibody detects FOXA1.
-
交叉反应 (详细)
-
Species reactivity (expected):Rat, Pig, Cow.
Species reactivity (tested):Human, Mouse. -
纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
-
免疫原
- Peptide from C-Terminus of the protein sequence according to NP_004487.2 Genename: FOXA1
-
-
-
-
应用备注
-
Peptide ELISA: Limit dilution 1/16000. Western blot: 0.2-0.6 μg/mL. Approx 50 kDa band observed in Human lysates of cell lineHepG2 (calculated MW of 49.0 kDa according to NP_004487.2). An additional band ofunknown identity was also consistently observed at 30 kDa. This band was successfullyblocked by incubation with the immunising peptide. Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Anonymous customers used this product successfully in ChIP with MCF7.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
-
-
-
浓度
- 0.5 mg/mL
-
缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Avoid repeated freezing and thawing.
-
储存条件
- 4 °C/-20 °C
-
储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
-
-
- FOXA1 (Forkhead Box A1 (FOXA1))
-
别名
- FOXA1 / TCF3A
-
背景
- FOXA1 is a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.Synonyms: Forkhead box protein A1, HNF-3-alpha, HNF-3A, HNF3A, Hepatocyte nuclear factor 3-alpha, TCF-3A, Transcription factor 3A
-
分子量
- 49.1 kDa (NP_004487.2)
-
基因ID
- 3169
-
NCBI登录号
- NP_004487
-
UniProt
- P55317
-
途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
抗原
-