NUP50 抗体 (C-Term)
Quick Overview for NUP50 抗体 (C-Term) (ABIN374317)
抗原
See all NUP50 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-ELHKILLEKK DA
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特异性
- This antibody detects NUP50 at C-term. It is expected to recognise both human isoforms of this protein.
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交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human. -
纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_009103.2, NP_705931.1. Genename: NUP50
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anti-Nucleoporin 50kDa (NUP50) (AA 37-269) antibody
NUP50 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (AA 1-468) antibodyNUP50 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (C-Term) antibodyNUP50 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) antibodyNUP50 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (C-Term) antibodyVerified NUP50 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (AA 342-439) antibodyNUP50 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4H7 unconjugated
anti-Nucleoporin 50kDa (NUP50) (AA 299-468) antibodyNUP50 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (AA 179-428) antibodyNUP50 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (AA 432-461) antibodyNUP50 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Nucleoporin 50kDa (NUP50) (AA 351-450) antibodyNUP50 适用: 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.2-1 μg/mL. Approx 60 kDa band seen in Jurkat lysate (predicted MW 54 kDaaccording to NP_009103).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- NUP50 (Nucleoporin 50kDa (NUP50))
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别名
- NUP50
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背景
- The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.Synonyms: NPAP60L, Nuclear pore-associated protein 60 kDa-like, Nucleoporin 50 kDa
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基因ID
- 10762
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NCBI登录号
- NP_009103
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UniProt
- Q9UKX7
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途径
- Tube Formation
抗原
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