ALMS1 抗体 (C-Term)
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Quick Overview for ALMS1 抗体 (C-Term) (ABIN374311)
抗原
See all ALMS1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-RVTNQLLGRK VPWD
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特异性
- This antibody detects ALMS1 at C-term.
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交叉反应 （详细）
- Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_055935.4 Genename: ALMS1
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anti-Alstrom Syndrome 1 (ALMS1) (C-Term) antibody
Verified ALMS1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Alstrom Syndrome 1 (ALMS1) (C-Term) antibodyALMS1 适用: 人, 大鼠 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alstrom Syndrome 1 (ALMS1) (Internal Region) antibodyALMS1 适用: 人 ELISA, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Alstrom Syndrome 1 (ALMS1) (AA 889-1057) antibodyALMS1 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 32000. Western blot: Preliminary experiments gave bands at approx 200 kDa and 70 kDa in lysatesof Human T cell line Jurkat after 0.3 μg/mL antibody staining. Please note that currently wecannot find an explanation in the literature for the bands we observe given the calculatedsize of 460 kDa according to NP_055935.3. Both detected bands were successfully blockedby incubation with the immunizing peptide (and BLAST results with the immunizingpeptide sequence did not identify any other proteins to explain the additional bands). Wewould appreciate any feedback from people in the field - have any results been reportedwith other antibodies/lysates? Have any further splice variants/modified forms beenreported?Immunohistochemistry: 1-3 μg/mL. In paraffin embedded Human Testis shows staining ofcytoplasm in spermatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- ALMS1 (Alstrom Syndrome 1 (ALMS1))
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别名
- ALMS1
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背景
- ALMS1 contains a large tandem-repeat domain that contains no cysteine residues. Mutations in this gene have been associated with Alstrom syndrome. The encoded protein may play a role in hearing, sight, obesity, and liver function. Alternative splice variants have been described but their full length sequences have not been determined.Synonyms: Alstrom syndrome protein 1, KIAA0328
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基因ID
- 7840
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NCBI登录号
- NP_055935
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UniProt
- Q8TCU4
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途径
- Sensory Perception of Sound, Carbohydrate Homeostasis, M Phase
抗原
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