Midline 2 抗体 (C-Term)
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Quick Overview for Midline 2 抗体 (C-Term) (ABIN374299)
抗原
See all Midline 2 (MID2) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- QESPYVSGMK TCH
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特异性
- This antibody detects RNF60 / MID2 at C-term.
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交叉反应 （详细）
- Species reactivity (expected):Rat, Dog, Pig, CowSpecies reactivity (tested):Human, Mouse
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纯化方法
- Amonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_036348.2, NP_438112.2 Genename: MID2
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anti-Midline 2 (MID2) (AA 506-735) antibody
MID2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (C-Term) antibodyVerified MID2 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 251-350) antibodyMID2 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 690-717), (C-Term) antibodyMID2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB33238 unconjugated
anti-Midline 2 (MID2) (AA 1-685) antibodyMID2 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Midline 2 (MID2) (C-Term) antibodyMID2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 210-259) antibodyMID2 适用: 人, 小鼠, 大鼠, 犬, 兔, 猴, 豚鼠, 马, Pig, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Midline 2 (MID2) (AA 690-717), (C-Term) antibodyMID2 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Lmit dilution 1: 32000. Western bot: 1-3 μml. Approx 80 kDa band observed in mouse heart lysates (calculated MWof 81.0 kDa according to NP_036348.1 and 77.0 kDa according to NP_438112.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows strongstaining in cytoplasm and brush border of some epithelial cells of proximal convolutedtubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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