Peptide ELISA: Lmit dilution 1: 32000. Western bot: 1-3 μml. Approx 80 kDa band observed in mouse heart lysates (calculated MWof 81.0 kDa according to NP_036348.1 and 77.0 kDa according to NP_438112.1). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Kidney shows strongstaining in cytoplasm and brush border of some epithelial cells of proximal convolutedtubules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.