BAZ2B 抗体 (C-Term)
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Quick Overview for BAZ2B 抗体 (C-Term) (ABIN374295)
抗原
See all BAZ2B 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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序列
- C-YFEKKWTDTF KVS
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特异性
- This antibody detects BAZ2B at C-term.
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交叉反应 （详细）
- Species reactivity (expected):Mouse, Rat, CowSpecies reactivity (tested):Human
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纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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免疫原
- Peptide from C Terminus of the protein sequence according to NP_038478.2 Genename: BAZ2B
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anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (AA 1833-1931) antibody
BAZ2B 适用: 人 ELISA, WB, IHC (p) 宿主: 小鼠 Monoclonal 2D10 unconjugated
anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (AA 139-240) antibodyBAZ2B 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (AA 1-643) antibodyBAZ2B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B) (C-Term) antibodyVerified BAZ2B 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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应用备注
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Peptide ELISA: Limit dilution 1: 4000. Western blot: Preliminary experiments gave an approx 35 kDa band in Testis and Brainlysates after 0.3 μg/mL antibody staining. Please note that currently we cannot find anexplanation in the literature for the band we observe given the calculated size of 225 kDaaccording to NP_038478. The 35 kDa band was successfully blocked by incubation with theimmunizing peptide. We would appreciate any feedback from people in the field - have anyresults been reported with other antibodies/lysates? Have any further splicevariants/modified forms been reported?Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Small Intestine showsprimarily nuclear staining in a number of cells of the lamina propria.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- BAZ2B (Bromodomain Adjacent To Zinc Finger Domain, 2B (BAZ2B))
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别名
- BAZ2B
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背景
- BAZ2B may play a role in transcriptional regulation interacting with ISWI. It is expressed at varying levels in several tissues, whereas a smaller transcript was expressed specifically in testis.Synonyms: Bromodomain adjacent to zinc finger domain protein 2B, KIAA1476, hWALp4
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基因ID
- 29994
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NCBI登录号
- NP_038478
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UniProt
- Q9UIF8
抗原
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